Join us virtually or in-person in Boston, MA for the 14th annual Bioprocessing Summit where Just-Evotec Biologics will be presenting.
- Todd Ireland, Senior Director Business Development at Just-Evotec Biologics
- Nina Reese, Scientist, Cell Line Development at Just-Evotec Biologics
Presentation | 16 August at 9:00am ET
Development of Recombinase-Based Targeted Integration Systems for Production of Exogenous Proteins Using Transposon-Mediated Landing Pads
Current methods for stably expressing biotherapeutics in CHO cells often rely on random or semi-random integration events which result in widely heterogeneous cell populations. In this study, we developed a targeted integration system that expresses recombinant proteins using transposon-mediated landing pads. By targeting predefined genomic locations that support high expression of exogenous proteins, several cell lines expressing different biotherapeutics can be established with a high degree of specificity and reproducibility.
Presented by Nina Reese, Scientist, Cell Line Development at Just-Evotec Biologics
Nina ReeseScientist, Cell Line Development
Nina Reese is a process development scientist in the cell line development group at Just Evotec Biologics. During her 5 years at Just, she has focused on the development of several internal CHO host cell lines. Her work includes the generation of a targeted integration system for the expression of recombinant therapeutic proteins as well as the creation of CHO knockout host cell lines using CRISPR-like technology. She has also led the development of multiple cell lines expressing therapeutic drug candidates. Prior to joining Just, Nina received her bachelor’s degree in bioengineering from the University of Washington and researched the transcriptional activation of latent HIV.