70th Annual ASMS – 2022
Join us in Minneapolis, Minnesota for the 70th ASMS Conference on Mass Spectrometry and Allied Topics.
- Rosalynn Molden, Senior Scientist at Just-Evotec Biologics
- Erin Weisenhorn, Ph.D., Senior Scientist at Just-Evotec Biologics
- Yuko Ogata, Senior Scientist at Just-Evotec Biologics
Distinguishing between mAb clipping sites and spurious trypsin activity during peptide mapping
Traditional methods such as rCE-SDS and SDS-PAGE are used to identify therapeutic monoclonal antibody low molecular weight species (LMW). These LMW species may be caused by disulfide bond disruption or by clipping within the protein backbone by proteases or non-enzymatic fragmentation. Mass spectrometry is an important method to identify the exact location of the clip within the antibody sequence. Reduced peptide mapping is a sensitive method that can be used to identify clips, but pre-existing clips in the sequence need to be distinguished from cuts made by the protease used to perform the digestion.
Presented by Rosalynn Molden, Senior Scientist at Just-Evotec Biologics
Metabolomic Characterization of CHO media and lysate during production of biotherapeutics
The production of monoclonal antibodies for biotherapeutics is frequently performed in a Chinese Hamster Ovary (CHO) cell line due to similarity to human glycosylation patterns and high expression. These cell lines are expertly engineered and selected to maximize antibody titer and manufacturability. As such, significant perturbations to normal metabolism occur during the production of antibodies in bioreactors. Protein sequence, cell line, clones, and media may alter the stress experienced by cells during production and therefore inform opportunities to optimize the culture for maximum viability and titer. Antibody titer has one of the largest impacts on determining manufacturing for protein biotherapeutics and thus can be key in determining the price point and accessibility of new biologic treatments.
Presented by Erin Weisenhorn, Ph.D., Senior Scientist at Just-Evotec Biologics
New Aspects in the Development of Multi-Attribute Method (MAM)
Yuko Ogata, Senior Scientist at Just-Evotec Biologics