Current methods for stably expressing biotherapeutics in CHO cells often rely on random or semi-random integration events which result in widely heterogeneous cell populations. In this study, we developed a targeted integration system that expresses recombinant proteins using transposon-mediated landing pads. By targeting predefined genomic locations that support high expression of exogenous proteins, several cell lines expressing different biotherapeutics can be established with a high degree of specificity and reproducibility.
Read the full study in the Current Research in Biotechnology (CRBIOT) Journal.